根據對蘋果器官的緻病性差異可将蘋果炭疽葉枯病劃分為兩種緻病類型：苦腐型（ABR pathotype）和葉枯型（GLS pathotype）。
苦腐型僅侵染近成熟果實，引起典型腐爛症狀，但不侵染葉片，對品種無選擇性，該類病害通常稱為苦腐病（apple bitter rot，ABR），或蘋果炭疽病（Southworth 1891；Sutton 1990）。
蘋果
葉枯型能侵染葉片和幼果，對品種有嚴格的選擇性，主要危害嘎拉系、金冠系和元帥系蘋果品種，引起炭疽葉枯病（Leite et al. 1988；Sutton & Sanhueza 1998）。
本研究通過基因功能證明Cfcyp450是一個葉枯型菌株關鍵緻病基因。同源性比對顯示C. fructicola葉枯型基因Cfcyp450與C. aenigma葉枯型基因相似度為100%，為同源基因，但在C. fructicola苦腐型菌株基因組中不存在該基因。
蘋果
此外Cfcyp450與已知煙草内生真菌Sodiomyces alkalinus F11和拟南芥内生真菌Dactylonectria macrodidyma同源性相對較高（劉宏玉 2014； Grum-Grzhimaylo et al. 2018；Habibi & Ghaderi 2020；Kuvarina et al. 2021），與刺盤孢屬真菌親緣關系較遠，同源性低。
這些結果暗示苦腐型菌株可能通過一些内生真菌的基因水準轉移獲得能侵染葉片的獨特基因，進而變成葉枯型菌株，該假設有待進一步的試驗驗證。
真菌的P450基因為一類重要緻病基因，可通過參與次級代謝産物的合成來影響真菌的緻病 性。
最近對植物病原菌CYP基因的功能分析中發現一些新的CYP基因，涉及毒性、無性發育和有性發育以及外源生物的降解。灰葡萄孢Botrytis cinerea P450單加氧酶基因bcbot1在植物中表達，對B. cinerea的侵染發揮重要作用（Verena et al. 2005）。
蘋果
大麗輪枝菌Verticillium dahliae的T-DNA突變體M01C06緻病性下降，其單加氧酶基因VdCYP1是關鍵緻病相關因子，可能通過參與大麗輪枝菌的次級代謝影響緻病過程（Zhang et al. 2016）。
稻瘟菌Magnaporthe oryzae中鑒定到MOMCP1，通過影響附着胞的形成而增強緻病力( Wangetal.2019)。尖豌豆專化型Foxysporum f.sp.pisi 晚豆素脫甲基酶FOPDA能夠對植保索豆素進行解毒促進其侵染( Georgeet al. 1998;Coleman et al.2011)。
本研究顯示果生刺盤中Cfcyp450基因含有保守的CYP450結構域，通過影響附着胞的形成及穿透而影響緻病力，是否參與解毒作用、次級代謝産物的合成來影響真菌的緻病性尚有待進一步研究。
果生刺盤抱為半活體營養菌。分生抱子萌發産生芽管，芽管末端膨大形成附着胞，附着胞通過産生侵染釘穿透寄主角質層和表皮細胞，分化形成活體初生侵染菌絲，初生侵染菌絲随後分化形成次生侵染菌絲，以死體營養方式在組織内擴充( Crusius etal. 2002; Shang etal.2020)。
果生刺盤抱為一種寄主範圍非常廣泛的病原菌，近年來對其緻病基因功能進行了許多研究。油茶果生刺盤抱 bZIP 轉錄因子 CfHacI 和自噬相關因子 CfAtg8 參與調控 ccticola 的生長發育、附着胞的形成、緻病力及響應外界滲透壓的脅迫過程(姚權等 2019: 郭源等 2020)。
果炭疽葉枯病病原果生刺盤抱轉錄因子 CfStel2 基因缺失後其分生子萌發率、附着胞形成率顯著下降并喪失對嘎拉葉片和果實的緻病性(Liu etal.2020)。C. ucticola 的 MAPK 基因 CfPMKI敲除突變體不能分化形成附着胞，是以不能穿透角質層，緻病力顯著下降 ( Liang et al 2019)。
C.fructicola 的 MADS-box 轉錄因子 CMcmI調控緻病性分生抱子萌發和性發育(Liu etal.2022)。本研究中發現蘋果果生刺盤抱 Cfcyp450 缺失突變體産抱量下降、附着胞的萌發和穿透降低，緻病力顯著減弱，我們推測 Cfcyp450 通過影響果生刺盤抱附着胞的萌發和穿透，導緻其緻病力減弱。有關 Cfcyp450對緻病性的具體機制還有待進一步的探索。
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