引言（来源于ChatGPT）
Cytogenetic engineering is a fast-growing field that involves techniques such as genome editing, gene transfer, and cell reprogramming with the aim of improving the properties or functions of living organisms. The following are some of the research hotspots in this field and their future development directions:
Research hotspots
1. Advances in genome editing technology
- CRISPR-Cas9 system: CRISPR-Cas9 has become a core tool in cytogenetic engineering, with researchers improving its accuracy and efficiency, such as developing novel sgRNA designs and variants (e.g., CRISPR-Cas12 and Cas13).
- Safety of gene editing: Investigate how to reduce the off-target effects of genome editing and develop new assays to avoid potential genetic risks.
2. Cell reprogramming
- Induced pluripotent stem cells (iPSCs): Research on the use of adult cells to induce reprogramming into iPSCs by transcription factors has increased the potential for application in regenerative medicine and disease modeling.
- Cell Fate Shift: Develop new methods and strategies to transform one cell type into another, addressing issues such as organ regeneration and repair.
3. Synthetic Biology
- Application of bioinformatics and computational tools: Use big data, machine learning and other technologies to optimize gene design and system construction, and promote the development and application of new biological systems.
- Biosynthetic pathway design: Engineering cells and microorganisms to build new metabolic pathways to synthesize drugs, fuels, and other high-value compounds.
4. Cell therapy vs. gene therapy
- CAR-T cell therapy: Activates the patient's own immune cells to attack cancer cells, and delves into how to improve their safety and efficacy.
- Gene Replacement Therapy: Investigating innovative solutions for the treatment of genetic diseases through pathological gene modification or replacement.
5. The gut microbiome and human health
- Cell-Microbial Interactions: Studying the co-interaction of gut cells and microbes and how cytogenetic engineering can improve the impact of the microbiome on host health.
Future directions
1. Precision medicine
- Combining genome editing with personalized patient medicine, we can provide personalized treatment plans through accurate analysis of patients' genomes to improve efficacy and reduce side effects.
2. Ethical and social issues
- With the popularization of cytogenetic engineering technology, research involving ethical, legal and social issues will become an important direction to ensure the safe use and reasonable regulation of technology.
3. New carriers and delivery systems
- Develop more efficient gene vectors and delivery systems, such as nanoparticles, viral vectors, etc., to improve the efficiency and safety of gene transfer.
4. Biomaterials and Regenerative Medicine
- Cytogenetic engineering will play an increasingly important role in tissue engineering and regenerative medicine, promoting the construction of novel tissues or organs by combining with biomaterials.
5. Interdisciplinary research
- Intersect with other disciplines such as physics, chemistry, and computer science to promote technological progress in cytogenetic engineering and generate new and innovative applications.
conclusion
As an important part of the biotechnology field, cytogenetic engineering is constantly pushing the boundaries of tradition and promoting the development of biomedicine, agriculture and environmental science. In the future, with the advancement of technology and the improvement of social acceptance, cytogenetic engineering will bring more positive impacts on human health, food safety and environmental protection.
Big data analytics
检索数据库：Medline
检索工具：文献鸟/PubMed
Search time: 2024-09-28
检索词：(genetic modification OR genetic engineering) AND China[ad]
1. Overview of the paper
In recent years, China has published 72,402 research articles related to cytogenetic engineering included in Medline, and we conducted big data analysis on 9,999 articles included in it, including 2,917 articles published in 2023 and 7,054 articles newly published in 2024. Use ChatGPT to learn more about the research hotspots of cytogenetic engineering.
2. Active institutions in China for cytogenetic engineering research: Fudan University published 371 papers, Zhejiang University (174 papers), Jiangnan University (169 papers), China Agricultural University (153 papers), Huazhong Agricultural University (136 papers), Central South University (133 papers), Sichuan University (120 papers), etc.
3. Hospitals with active publications in China on cytogenetic engineering research: Zhongshan Hospital published 74 papers, West China Hospital (67 papers), Fudan University Cancer Hospital (55 papers), Huashan Hospital (42 papers), Xiangya Hospital (37 papers), Renji Hospital (29 papers), The First Affiliated Hospital of Zhengzhou University (27 papers), Zhongnan Hospital of Wuhan University (24 papers), Children's Hospital Affiliated to Fudan University (23 papers), etc.
4. Cytogenetic Engineering Research Chinese Authors Published Journals:
从发文来看，发表来自中国细胞遗传工程研究文章数量较多的期刊有Int J Mol Sci (IF: 4.9) (255篇)、Nat Commun (IF: 14.7) (207篇)、Front Plant Sci (IF: 4.1) (163篇)、J Agric Food Chem (IF: 5.7) (159篇)、Int J Biol Macromol (IF: 7.7) (159篇)、Sci Rep (IF: 3.8) (141篇)、Plants (Basel) (IF: 2.8) (121篇)、BMC Genomics (IF: 3.5) (105篇)等。
5. Cytogenetic engineering research: active scholars and their networks in China
Active experts in cytogenetic engineering research in China: Liu, Yang, Chinese Academy of Fishery Sciences; Zhang, Wei, Dali University; Chen, Haiquan, Fudan University; Ding, Chen, Fudan University; Li and Wei of Beijing Forestry University are active in this research field. There are many more excellent researchers, and it is impossible to list them all due to space limitations.
Limitations of this data analysis:
A. This report is a visual report based on the PubMed database based on the PubMed database, based on the search results of the set search terms, within a limited time and number of documents.
B. The purpose of the big data analysis of the "Literaturebird" analysis tool is to present an overview of recent research in the field and is for academic communication purposes only; There is no ranking significance.
C. The statistical ranking of the results of the "Literature Bird" analysis tool on the active unit, author, etc., and only the number of papers in the unit where the first author's paper is located is counted; That is, after the paper is retrieved and downloaded, only the first author's unit is retained for each paper, and then the number of papers in each unit is counted. When there are different spellings of the same unit, PubMed treats it as two different units. In the same authorship arrangement, only the number of papers published by the first author and the last author is counted. If the author's name has a different spelling, it will be processed by the PubMed search platform according to the different authors.
D. The conclusion of this paper is completely from the "Literature Bird" analysis tool, due to the limitations of search terms, the scope of literature included in the search database and the search time, it does not represent the views of this journal, and the data content is likely to be inaccurate.