Item 1: Biomedicine - respiratory virus infection blocker project for prevention
Project 2: Cultivation of hydrangea fruiting bodies and development of mask products
Project 3: Production of phenethyl alcohol by microbial transformation
Project 4: Energy and Environmental Protection - Microbiology Bureau Warm Rot Technology
Project 5: Bioprocessing of isoprene
Item 6: Streptomyces biocontrogens W68 and its solid-state fermentation technology
Item 7: Preparation techniques for oral vaccines for aquaculture in aquatic products
Project 1: Biomedicine - Respiratory virus infection blocker project for prevention
Brief introduction of results
Taking the AW mechanism of inhibiting respiratory single-stranded RNA virus as the target, according to the known infection mechanism of respiratory-transmitted viruses such as coronavirus, influenza virus, rhinovirus, etc., the small molecule substances that can be closely bound to the active compound site of their surface ligand proteins are designed and screened, neutralize the binding site, resist the invasion of the virus as a competitive cell inhibitor, and develop appropriate formulation forms to implement airway protection for susceptible populations during epidemics.
Level of achievement and technical advantages
The transformation center has been consorted into a variety of sialic acid-based ligand hoes that mimic influenza of respiratory epithelial cells and certain coronaviruses (such as MERS), and a large number of saliva-derived oligosperns or glycopeptides can be synthesized through this synthesis protocol, from which inhibitors that block the entry of the virus into the cell can be screened. And compound other ingredients that have bacteriostatic and toxic and do not cause allergies and irritation. At present, a variety of antiviral drugs reported, including purification preparations of traditional Chinese medicines, compound acetylcysteine and other protective agents and antioxidant grins, are joint nebulization preparations that can quickly enter clinical trials, technical advantages: high-density cationic polysaccharides need to be jointly applied for and pushed in, and the market bond threshold is very high.
Project 2: Cultivation of Hydrangea fruiting bodies and development of mask products
Hydrangea is a valuable edible and medicinal mushroom, and the new large-scale cultivation of edible mushrooms is rich in P-dextran. Not only is it delicious, but it also has a variety of nutritional and health functions. The high-quality strains screened by the team have achieved fruiting body cultivation. In addition, on the basis of the evaluation of tyrosinase inhibition, antioxidant and moisturizing function of hydrangea fruiting body extract, a mask based on hydrangea extract was developed, which has a good moisturizing effect.
1. Screening of high-quality strains to achieve fruiting body cultivation;
2. Hydrangea extract suitable for adding to various cosmetics;
3. Moisturizing effect of mask products.
Sections required to complete pilot or industrial production tests
Clean water source, temperature, humidity, ventilation can be controlled
Project 3: Microbial transformation of phenethyl alcohol production
2-Phenethyl alcohol has a light, delicate and long-lasting rosette aroma. The project adopts microbial conversion method to produce phenethyl alcohol, and the production of phenethyl alcohol reaches the leading level in China.
1. By designing a self-balancing system of cofactors, multiple cofactors can be regenerated at the same time to achieve efficient biosynthesis of phenethyl alcohol;
2. The output and conversion rate of phenethyl alcohol have reached the leading level in China;
3. There is no problem of phenethyl alcohol inhibiting cell production, short growth cycle and low fermentation cost.
Form of Assignment
Patents + technical secrets
Project 4: Energy and Environmental Protection - Microbiology Bureau Warm Rot Technology
High-temperature decomposition technology is the use of high-temperature bacteria under high temperature conditions, fast growth rate, strong metabolic capacity, high activity of degrading enzymes, can accelerate the decomposition of organic materials, improve the degradation efficiency of organic matter, and at the same time inhibit the breeding of pathogens and viruses under high temperature conditions. Therefore, high-temperature fungus decomposition technology can have broad prospects in the treatment of human excreta and domestic waste, livestock and poultry waste, and agricultural organic waste utilization.
The principle of results and technical advantages
1, the use of high temperature fungus generation time is short, fast growth, high enzyme activity;
2. It can improve the degradation efficiency of organic matter and maintain high temperature for a long time; it can shorten the biotransformation cycle of materials;
3. Maintaining high temperature conditions for a long time (above 55 °C) can inhibit the breeding of pathogens and viruses.
Project 5: Biological synthesis of isoprene
Using glucose as raw material, the engineering strain of E. coli is used to produce isoprene by fermentation method. In turn, it can synthesize isoamyl rubber with industrial and pharmaceutical value.
Isoprene produces a strength of more than 2.75 g/L/h, a conversion rate of 0.27 g/g glucose, and a fermentation level of more than 60 g/L.
New synthetic pathways reduce production costs.
Patent + technology transfer
Project 6: Streptomyces biocontrovirus W68 and its solid fermentation technology
Streptomyces microflora W68 secretes a variety of antifungal substances such as chitinase and nephroxin. Its bacteriostatic spectrum is wide, which can effectively prevent and control a variety of plant fungal diseases. The unique solid fermentation process can efficiently produce stress-resistant flaccid seeds, and the shelf life of fungal products is long.
1. Broad bacteriostatic spectrum, can colonize in the soil and efficiently prevent and control a variety of plant fungal diseases;
2. Large-scale solid-state fermentation, production capacity of up to 1 billion / g, strong resistance to B-seeds, long shelf life of products, leading in China;
3. Only one product of actinomycetes has been produced and applied on a large scale, and the development of Streptomyces microflora will form the second actinomycete microbial preparation product in China.
Patents and technical secrets
Project 7: Preparation technology for oral vaccines in aquaculture of aquatic products
Using natural aquatic product bait microalgae as the host, it efficiently expresses fish and shrimp virus shell proteins or broad-spectrum antibacterial peptides, and prepares microalgae oral vaccines with antiviral or antibacterial functions for disease prevention and control in aquatic product farming.
1. The use of oral vaccines can reduce the use of antibiotics or chemical fungicides in the aquaculture process, and avoid antibiotic residues in aquatic products and water bodies;
2. Oral vaccines are more suitable for application in aquaculture than traditional injectable vaccines.
Technical secrets + patents