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From January 16 to 18, Zhuhai implemented the "three days and three tests" policy for nucleic acids of all employees, and a large screening race against the virus was quickly rolled out. According to the report, for three consecutive days, the nucleic acid test results of all employees in Zhuhai were negative, and the cumulative number of test samples was 8.3085 million.
At 8:00 p.m. on the 18th, nucleic acid sampling specimens from various regions began to arrive at Zhuhai People's Hospital one after another. South + Guan Mingrong shooting
How does such efficient nucleic acid testing work? How many professional processes does a proper "purple business card" on a mobile phone go through in the laboratory?
On the evening of January 18, the reporter came to Zhuhai People's Hospital. The hospital's nucleic acid testing base is the first urban nucleic acid testing base in Zhuhai, which was originally established to cope with large-scale nucleic acid screening. At present, the base has 17 amplification instruments, 6 extraction instruments, and 49 professional inspectors. Under full load operation, it can detect nearly 20,000 samples 24 hours a day.
These days, day and night reels have become the norm. Working an average of more than 14 hours a day, they are transformed into a group of "virus scouts" who are providing strong support for the prevention and control of the epidemic in Zhuhai.
In the specimen reception room, the staff began to count nervously. South + Guan Mingrong shooting
Efficient and precise
Members practice professional understanding
What are the processes required for nucleic acid testing?
"Strictly speaking, the complete process involves a lot of steps." Zhang Zhenlin introduced that the inspection process mainly includes five parts: specimen collection and transportation, laboratory testing, data reporting, specimen archiving, and medical waste treatment, and each part involves many subdivisions.
In the laboratory part, the inspectors operate in accordance with the assembly line, from sample reception and inventory, unpacking and sorting, to reagent preparation, sample addition, extraction, amplification, result analysis and data processing, each post has set up a group of fixed personnel, using three shifts to rotate, to ensure continuous operation around the clock.
"Three tests in three days" means that the test results must be produced within one day. Therefore, every link in the laboratory needs to be accurate.
Stacks of sampling lists, the workload of the staff is very large. South + Guan Mingrong shooting
For example, after the sample is sampled, nucleic acid extraction, this process takes about 24 minutes per batch, and then the sampler adds the nucleic acid extract to the amplification reagent, and then gives it to the colleague of the amplification post for on-the-machine amplification, the amplification time is about one hour and 15 minutes, and the results are analyzed after the amplification is completed, and then handed over to the data clerk for data reporting, specimen archiving, and individual specimens may also need to be reviewed. Calculated, it takes about 4 hours for a batch of samples to arrive in the laboratory and complete the final test.
"Large-scale nucleic acid screening consumes a lot of reagent consumables", at the scene, Zhang Zhenlin checked the stock of reagent consumables every once in a while in order to replenish in time without affecting the detection process.
Not only that, the detection process is interlinked, and the working group also maintains a professional tacit understanding.
In the reagent preparation room, the reagent group personnel to expand the reagent configuration and sub-packing, and then handed over to the sampling group to count the plate, a branch of reagents are neatly and orderly distributed to the test board, and then the amplification group for sample information programming, the sample is placed on the amplification instrument amplification, after the expansion of the end of the amplification results of the analysis.
"The whole link we have to do quality control, including three blank controls, one negative property control, one weak yang nature control, one yang nature control for evaluation", Zhang Zhenlin said, the assessment is related to the final test quality of the sample, the result will be submitted to the data group review and uploaded to the report, and then the specimen is archived.
At this point, a complete nucleic acid testing laboratory process is over.
In the specimen processing room, the staff is performing spot sampling (adding nucleic acid templates) and amplification. South + Guan Mingrong shooting
Continuous combat
The second half of the night is the main battlefield
The sudden outbreak of the epidemic has tested the endurance and confidence of every inspector.
Since January 14, Zhang Zhenlin's team has been working overtime, the prescribed 8-hour rotation system, many members have done more than ten hours, Zhang Zhenlin himself is no exception.
"The second half of the night is the main battlefield of our testers", Zhang Zhenlin said, because large-scale nucleic acid screening is carried out during the day, the number of people tested and the testing progress are different, so most of the samples will be delivered to the inspection center at night, after sorting and checking, the work of the testing personnel will begin, for several consecutive days, they all work until the early morning. It is understood that there are 22 sites in the city that meet the nucleic acid test, and such a situation has also recurred at other testing points.
The studio is separated by thick glass inside and outside, and the staff can only communicate face-to-face through the intercom. South + Guan Mingrong shooting
The specimen is fed into the testing machine and the staff begins to monitor the results. South + Guan Mingrong shooting
In addition, as the number of close contacts has increased and the scope of control has expanded, the residences of some testers have also been included, and they have not returned home for several days.
"A colleague just came out to work the day before, and found that his building was sealed, there was no place to go, and he came back and still worked overtime, so he arranged accommodation for them at the hospital." Zhang Zhenlin said.
After the 90s, Zhao Xiaoguang has been on the nucleic acid testing line during this time, and after graduating with a doctorate, he came to work in the hospital, and in a year and a half, he experienced two large-scale nucleic acid screenings in Zhuhai, and he compared himself to the "universal candidate" on the court.
"In the testing center, I can stay up late, generally on the night shift, the receiving post is short of people I go to the receiving post, the reagent post is short of people I will go to the reagent, where I need to go." Zhao Xiaoguang said.
The staff is busy. South + Guan Mingrong shooting
At present, the base has 17 amplification instruments, 6 extraction instruments, and 49 professional inspectors. Under full load operation, it can detect nearly 20,000 samples 24 hours a day. South + Guan Mingrong shooting
Lin Qiuping and Zhao Xiaoguang are similar in age, just pregnant, she still went to work for 4 consecutive days, and only went back to rest at 9 o'clock in the evening, "On the first day of the epidemic, everyone may be more busy, and by the fifth day, the whole process has been very smooth, everyone has more and more tacit understanding, and the detection speed is getting faster and faster." "Lin Qiuping."
After a busy night, finally finishing the specimens, the staff wrote down their wishes. South + Guan Mingrong shooting
【Text/Appearance】Southern Daily reporter Wu Guanlin
【Shooting/Editing】Southern Daily reporter Guan Mingrong
【Author】 Wu Guanlin; Guan Mingrong
【Source】 Southern Press Media Group South + client
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