The latest consensus interpretation | these dozen respiratory pathogens urgently need multiple nucleic acid testing

Acute respiratory infections

Acute respiratory infection is the most common infectious disease in children, and acute lower respiratory tract infection is the first cause of death in children under 5 years of age, which seriously threatens the life and health of children and causes a serious burden of disease on families and society [1].

Recently, the new crown epidemic has rebounded in China, and respiratory infections have been paid more and more attention. What are the advantages of respiratory nucleic acid testing? And how to choose the right nucleic acid testing products? Let's hear what the experts have to say.

On March 13, the "Expert Consensus on Nucleic Acid Detection of Pathogens of Respiratory Tract Infections in Children" compiled by experts in various fields in China was published in the core journal of pediatrics in China Practical Pediatrics Clinical Journal, which pointed out that PCR-based nucleic acid amplification technology has become the most common nucleic acid diagnosis method for nucleic acid detection of respiratory pathogens because of its high efficiency, flexibility, relatively simple operation and low cost.

The design of multiple targets targeting a variety of pathogens and the research and development and application of integrated equipment with nucleic acid extraction, amplification and result reading highlight the advantages of nucleic acid detection in multiple rapid detection of pathogens.

Viruses and atypical pathogens are caused

An important pathogen of acute respiratory infections in children

A domestic study found that nearly 90% of hospitalized children with acute respiratory infection have at least one virus or atypical pathogen positive [2], mainly manifested as human rhinovirus, parainfluenza, pneumonia, mycoplasma, adenovirus, human Boca virus, etc.

Another study also reported that viral and atypical pathogens were detected at more than 85% in children hospitalized for acute respiratory tract. Including human rhinovirus, respiratory syncytial virus, adenovirus, parainfluenza virus, Mycoplasma pneumoniae, influenza A virus, human metapneumovirus, influenza B virus, coronavirus, Boca virus, Chlamydia pneumoniae, etc., the proportion of mixed infections can reach 30% [3].

Advantages of nucleic acid detection of multiple pathogens

The consensus pointed out: "The rational use of multiple nucleic acid detection is not only conducive to the pathogenic auxiliary diagnosis of respiratory infections in children, but also provides strong support for the differential diagnosis of pathogens and the prevention and control of nosocomial infections." ”

What kind of child

Is multi-nucleic acid testing appropriate?

The consensus pointed out: "For those with emergency indications and all hospitalizations due to acute respiratory infectious diseases, new respiratory infection symptoms (with or without fever) during hospitalization, or unexplained respiratory distress, acute exacerbation of chronic cardiopulmonary diseases, blood diseases, and children with tumors with neutropenia/lack of fever, nucleic acid detection methods should be recommended for pathogenic diagnosis according to age, clinical characteristics and onset season, etc., and multiple nucleic acid tests can be used for differential diagnosis." For critically ill children with acute respiratory infections, multiplex nucleic acid testing is recommended. ”

What exactly are the clinical and patient needs?

Coincidentally, on November 5, 2021, initiated by the Scientific Branch of the Chinese Medical Association and the National Clinical Research Center for Respiratory Diseases, edited by Professor Xu Baoping, and co-compiled by 30 domestic experts in the fields of pediatric respiratory medicine, infectious diseases and laboratory, the popular science picture book "Exposing the "Tricksters" in the Respiratory Tract" published by the People's Medical Publishing House was officially released at the Beijing Great Wall Pediatric Forum.

At the press conference, Professor Shen Kunling, honorary director of the Science Branch of the Chinese Medical Association, said: "For the multiple nucleic acid testing of more than 10 respiratory viruses and atypical pathogens commonly seen in the clinic, it can not only clarify the pathogenic diagnosis, identify infectious diseases, achieve accurate and effective protection and treatment, but also reduce antibacterial drug abuse. ”

Consensus writing experts and picture book authors have proposed the importance of multiple nucleic acid detection, which shows that multiple nucleic acid detection products for more than 10 respiratory viruses and atypical pathogens commonly seen in clinical practice can truly meet the needs of clinical and patients.

"Demystifying the "Tricksters" in the Respiratory Tract" popular science picture book introduces pathogens

"Sea" mind manufacturing flagship multiple

Resbex

Multiplex test kit for 13 respiratory pathogens

It covers the 13 major viruses and atypical pathogens introduced by the consensus and all pathogens in popular science picture books to help clinical accurate diagnosis and treatment.

The Multiplexing Kit for 13 Respiratory Pathogens developed and produced by Haiershi Gene [National Machinery Injection 20183400518] uses a capillary electrophoresis fragment analysis method based on a generation sequencing platform to detect 13 common respiratory pathogens.

Product advantages and features

Broad Spectrum: Compatible with upper and lower respiratory tract specimens, covering 80% of respiratory infections with high positive rates and severe high-risk pathogens*.

Precision: Capillary electrophoresis fragment analysis method, unique single tube multiplexing technology, triple quality control and UDG enzyme anti-pollution system to ensure higher specificity and sensitivity.

【Reference】

[1] LiuL，OzaS，HoganD，et al. Global，regional，and national causes of under-5 mortality in 2000-15：an updated systematic analysis with implications for the Sustainable Development Goals[J]．Lancet，2016，388(163)：3027-3035. DOI：10.1016/S0140-6736(16)31593-8.

[2] Zhao M, Li G, Zhang D, et al. Diagnostic Microbiology and Infectious Disease, 2017, 88(2): 115-119.

[3] Wen S, Lv F, Chen X, et al. Journal of Medical Microbiology, 2019, 68(8): 1211-1218.

*Viruses and atypical pathogens

Edited by: Yeah Reviewer: Xiao Ran